Functional analysis of -351 interleukin-9 promoter polymorphism reveals an activator controlled by NF-κB

摘要: Genetic studies have shown linkages for asthma to the chromosomal region 5q31-q33 in humans that includes IL-9 gene. An A-to-G base substitution has been identified at bp -351 promoter. The role of this polymorphism promoter function was assessed utilizing CD4+ T cells purified from individuals with one or two G alleles comparison those homozygous wild-type A. presence an A (A allele) increased mitogen-stimulated transcription twofold subjects position. Binding nuclear extract proteins IL-9-producing human cell lines DNA sequences including exchange demonstrated specific binding factor NF-kappaB. NF-kappaB confirmed vivo using chromatin immunoprecipitation assay. Recombinant bound a fragment allele fivefold higher affinity than it did allele. Individuals carrying display synthesis IL-9, which may result strong Th2 immune responses and modulation their susceptibility infectious, neoplastic, parasitic atopic disease.