Human liver RNA-programmed in vitro synthesis of a polypeptide related to human apolipoprotein B
作者： Sven-Olof Olofsson , Per Elias , Kristina Boström , Kent Lundholm , Per-Ingvar Norfeldt
DOI: 10.1016/0014-5793(83)80249-X
关键词: PMSF 、 Apolipoprotein B 、 Polyacrylamide gel electrophoresis 、 In vitro 、 Low-density lipoprotein 、 Translation (biology) 、 Biology 、 Antiserum 、 Biochemistry 、 Molecular biology 、 RNA
摘要: Abstract In an in vitro synthesizing system programmed with RNA from human liver a polypeptide estimated M r of 80000 (80 kDa)±1400 (mean±SD, n =5) was synthesized. This could be precipitated antiserum to narrow density cut LDL ( d =1.030-1.055) or against the high- form apoB (apoB 100 [4]). The synthesized protein is immunologically related 75 kDa isolated LDL. We suggest that 80 represents primary translation product liver.
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