Neuro–glia interaction effects on GFAP gene: a novel role for transforming growth factor-β1

摘要: Central nervous system (CNS) development is highly guided by microenvironment cues specially provided neuron‐glia interactions. By using a transgenic mouse bearing part of the gene promoter astrocytic maturation marker GFAP (glial fibrillary acidic protein) linked to b-galactosidase (b-Gal) reporter gene, we previously demonstrated that cerebral cortical neurons increase b-Gal astrocyte number and activate secretion soluble factors in vitro. Here, identified TGF-b1 as major mediator this event. Identification neuronal extracts revealed both cell types might synthesize factor, however, addition monolayers greatly increased synthesis astrocytes. Further, exploiting advantages culture investigated influence neuron developmental stage on such interaction. We younger derived from 14 embryonic days wild-type mice were more efficient promoting differentiation than those 18 mice. Similarly, astrocytes also exhibited timed-schedule developed responsiveness with being responsive newborn late postnatal RT-PCR assays transcripts young but not old neurons, suggesting inability induce related secretion. Our work reveals an important role for interactions strongly implicates involvement