Encephalitogenic T cells use LFA-1 for transendothelial migration but not during capture and initial adhesion strengthening in healthy spinal cord microvessels in vivo.

摘要: LFA-1 on the surface of encephalitogenic T cells has been suggested to be involved in pathogenesis experimental autoimmune encephalomyelitis. By applying a novel technique intravitalfluorescence microscopy that enables us visualize interaction circulating T lymphoblasts within healthy spinal cord white matter microvasculature vivo, weinvestigated possible involvement their multi-step with blood-brain barrier endothelium vivo. was found mediate neither G-protein-independent capture nor G-protein-dependent initial adhesion strengthening T cell blasts microvessels. In contrast, blocking resulted significantly reduced number firmly adhering microvessels 2 h after injection and subsequently migrating across vascular wall into parenchyme. Our study provides first direct evidence use for transendothelial migration but not