Gene-expression profiling of HIV-1 infection and perinatal transmission in Botswana.

摘要: Perinatal transmission of human immunodeficiency virus (HIV)-1 represents a major problem in many regions the world, especially Southern Africa. With exception viral and proviral load, role for maternal cofactors perinatal outcome is largely unknown. In this study, an assessment was made peripheral blood mononuclear cells (PBMC) gene-expression profiles to better understand transcriptional changes associated with HIV-1 infection among young adult mothers infants Botswana. Peripheral specimens were used from 25 HIV+ drug naive 20 HIV− healthy mothers, similar age location, collected 1999–2000 2003, processed exact same methods, as previously described. Expression profiling 22 277 microarray gene probes implicated broad initiation innate response gene-sets, including toll-like receptor, interferon-stimulated antiviral RNA pathways association infection. Maternal status further host genes that influence processing splicing patterns. addition real-time polymerase chain reaction validation specific genes, enriched category PBMC conducted using two independent data sets either or unrelated virus, severe acute respiratory pathogen-specific should prove useful tool intervention efforts worldwide.