Effect of docosahexaenoic acid and furan fatty acids on cytokinesis block micronucleus cytome assay biomarkers in astrocytoma cell lines under conditions of oxidative stress

摘要: Fatty acids from fish such as docosahexaenoic acid (DHA) are associated with improved brain function, whereas furan fatty (FFAs) also found in oil at low levels (1%) thought to have antioxidant properties. Understanding their effects astrocytes is important these cells responsible for maintaining healthy neurons via lipid homeostasis and distribution within the brain, decline aging a possible cause of dementia. We investigated cytotoxic genotoxic DHA FFA using cytokinesis-block micronucleus cytome assay vitro cultures U87MG (APOE ɛ3/ɛ3) U118MG ɛ2/ɛ4) astrocytoma cell lines without hydrogen peroxide (H2O2, 100 µM) challenge. was be more sensitive cytostatic, (i.e., apoptosis), DNA damaging [micronuclei (MNi), nucleoplasmic bridges (NPBs), nuclear buds (NBUDs)] H2O2 (P < 0.01 P < 0.001) when compared U87MG. µg/mL significantly affected cytostasis (P < 0.05) increased damage form NPBs MNi both lines, it decreased necrosis (P = 0.0251) Significant DHA–H2O2 interactions were observed (P = 0.0033) biomarkers (P < 0.0001) line line. The varied between significant (P = 0.0022) line, increasing (P = 0.0144) Overall, exerted minimal on biomarkers. Environ. Mol. Mutagen. 55:573–590, 2014. © 2014 Wiley Periodicals, Inc.