Enzymatic manipulations of DNA oligonucleotides on microgel: towards development of DNA–microgel bioassays
作者： Md Monsur Ali , Shunxing Su , Carlos D. M. Filipe , Robert Pelton , Yingfu Li
DOI: 10.1039/B709817K
关键词: DNA polymerase 、 Polymerase cycling assembly 、 Biochemistry 、 DNA 、 Chemistry 、 Oligonucleotide 、 Hybridization probe 、 DNA ligase 、 Real-time polymerase chain reaction 、 Rolling circle replication
摘要: We demonstrate that DNA oligonucleotides covalently coupled to colloidal microgel can be manipulated by T4 ligase for ligation and Phi29 polymerase rolling circle amplification (RCA). also show the long single-stranded RCA product generate intensive fluorescence upon hybridization with complementary fluorescent probe. believe DNA-microgel conjugates explored development of based bioassays biosensors.
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