Development of RNAi Methods for Peregrinus maidis, the Corn Planthopper

摘要: The corn planthopper, Peregrinus maidis, is a major pest of agronomically-important crops. maidis has large geographical distribution and transmits Maize mosaic rhabdovirus (MMV) stripe tenuivirus (MSpV). objective this study was to develop effective RNAi methods for P. maidis. Vacuolar-ATPase (V-ATPase) an essential enzyme hydrolysis ATP transport protons out cells thereby maintaining membrane ion balance, it been demonstrated be efficacious target in other insects. In study, two genes encoding subunits V-ATPase (V-ATPase B D) were chosen as genes. open reading frames D generated used constructing dsRNA fragments. Experiments conducted using oral delivery microinjection investigate the effectiveness Real-time quantitative reverse transcriptase-PCR (qRT-PCR) analysis indicated that led minimum reduction 27-fold normalized abundance transcripts days post injection, while ingestion resulted two-fold after six feeding. While both knockdown transcripts, injection method more rapid effective. transcript observable phenotypes. Specifically, development nymphs injected with 200 ng either or impaired, resulting higher mortality lower fecundity than control insects GFP dsRNA. Microscopic examination these revealed female reproductive organs did not normally. successful specific will enable new insect strategies functional vital associated interactions between MMV.