Altered ganglioside composition in virally transformed rat embryo fibroblasts.
作者： Hongwei Bai , Joseph Orlando , Thomas N. Seyfried
DOI: 10.1016/0167-4889(92)90080-U
关键词: Biochemistry 、 Molecular biology 、 Cell culture 、 Sialic acid 、 Thin-layer chromatography 、 Fibroblast 、 Embryo 、 Cell division 、 Transformation (genetics) 、 Ganglioside 、 Biology
摘要: The composition of gangliosides was examined in a normal rat embryo fibroblast cell line (REF52) and two viral transformants: polyoma transformant (REF52-PyMLV) simian 40 (REF52-SV40). distribution the lines determined using gas-liquid chromatography high-performance thin-layer chromatography. N-acetylneuraminic acid predominant sialic species detected three lines. total ganglioside concentration (microgram/100 mg dry weight cells) normal, PyMLV, SV40 144.7 +/- 10.4, 153.8 9.2, 86.1 6.8, respectively. Gangliosides GM3, GM2, GM1, GD1a were major transformed these gangliosides, however, differed markedly between also themselves. cells from growth rate, morphology, social behavior. with highest GM3 content (PyMLV) formed islands, whereas lines, which had lower levels, grew as monolayers. findings suggest that PyMLV transformation can have multiple different effects on cellular
elsevier.com
sci-hub.se 参考文章(30)
S Ando, R K Yu, Isolation and characterization of a novel trisialoganglioside, GT1a, from human brain. Journal of Biological Chemistry. ,vol. 252, pp. 6247- 6250 ,(1977) , 10.1016/S0021-9258(17)39945-3
S. Ando, R.K. Yu, Isolation and characterization of two isomers of brain tetrasialogangliosides. Journal of Biological Chemistry. ,vol. 254, pp. 12224- 12229 ,(1979) , 10.1016/S0021-9258(19)86453-0
P L Coleman, P H Fishman, R O Brady, G J Todaro, Altered ganglioside biosynthesis in mouse cell cultures following transformation with chemical carcinogens and x-irradiation. Journal of Biological Chemistry. ,vol. 250, pp. 55- 60 ,(1975) , 10.1016/S0021-9258(19)41978-9
E G Bremer, S Hakomori, D F Bowen-Pope, E Raines, R Ross, Ganglioside-mediated modulation of cell growth, growth factor binding, and receptor phosphorylation. Journal of Biological Chemistry. ,vol. 259, pp. 6818- 6825 ,(1984) , 10.1016/S0021-9258(17)39801-0
Lars Svennerholm, Quantitative estimation of sialic acids. II. A colorimetric resorcinol-hydrochloric acid method. Biochimica et Biophysica Acta. ,vol. 24, pp. 604- 611 ,(1957) , 10.1016/0006-3002(57)90254-8
G. Yogeeswaran, Rose Sheinin, John R. Wherrett, Robert K. Murray, Studies on the Glycosphingolipids of Normal and Virally Transformed 3T3 Mouse Fibroblasts Journal of Biological Chemistry. ,vol. 247, pp. 5146- 5158 ,(1972) , 10.1016/S0021-9258(19)44950-8
Robert W. Ledeen, Robert K. Yu, Gangliosides: structure, isolation, and analysis. Methods in Enzymology. ,vol. 83, pp. 139- 191 ,(1982) , 10.1016/0076-6879(82)83012-7
Sen-itiroh Hakomori, Carol Teather, Henry Andrews, Organizational difference of cell surface “hematoside” in normal and virally transformed cells Biochemical and Biophysical Research Communications. ,vol. 33, pp. 563- 568 ,(1968) , 10.1016/0006-291X(68)90332-X
Taki Takao, Hirabayashi Yoshio, Ishiwata Yoshiro, Matsumoto Makoto, Kojima Kiyohide, Biosynthesis of different gangliosides in two types of rat ascites hepatoma cells with different degrees of cell adhesiveness Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. ,vol. 572, pp. 113- 120 ,(1979) , 10.1016/0005-2760(79)90205-4
Hirabayashi Yoshio, Taki Takao, Matsumoto Makoto, Kojima Kiyohide, Comparative study on glycolipid composition between two cell types of rat ascites hepatoma cells. Biochimica et Biophysica Acta. ,vol. 529, pp. 96- 105 ,(1978) , 10.1016/0005-2760(78)90107-8