Modulation of cell phenotype in human osteoblast-like cells by the simian virus 40

摘要: At present, the majority of in vitro research into bone metabolism is performed on either primary cultures or osteosarcoma lines. A better model behaviour normal cells would be a cell line derived from normal, adult that retained osteoblast-like characteristics. We infected culture humans with simian virus 1613, variant 40, and obtained 12 clones variable morphology. The were maintained for as long 6 months. Population doubling times, synthesis alkaline phosphatase osteocalcin, secretion mineral, morphology, ability to withstand freezing examined. SV/EC morphology varied polygonal, bipolar, fibroblast-like. times ranged 0.55 2.8 days (compared 3.9 nontransformed human cells). Synthesis but was less than by cells. With exception clone 11, all transformed synthesised mineral under mineralising conditions. Four showed increased population after passaging. All successfully frozen thawed, but, unlike cells, none responded stimulation parathyroid hormone. These results demonstrate can incorporate 1613 while retaining some their Such manipulation affords these an extended lifespan, this, together freezing, makes them potentially useful tool research.