Methods and compositions for enhanced protein expression and/or growth of cultured cells using co-transcription of a Bcl2 encoding nucleic acid

作者: Celia Ly , ChiChang Lee , Gordon Moore , Xiaomei Shi

DOI:

关键词: Protein expressionBiochemistryNucleic acidEnhanced growthProtein biosynthesisCo transcriptionHost (biology)Biology

摘要: The present invention in the field of biotechnology, provides methods and compositions for providing enhanced growth of, and/or protein production from, cultured mammalian host cells used commercially useful amounts expressed proteins, by use at least one Bcl2 encoding nucleic acid provided or transcribed cell.

参考文章(40)
D. Fassnacht, S. Rössing, R. P. Singh, M. Al-Rubeai, R. Pörtner, Influence of bcl-2 on antibody productivity in high cell density perfusion cultures of hybridoma. Cytotechnology. ,vol. 30, pp. 95- 106 ,(1999) , 10.1023/A:1008055702079
Alan Leatham, Andrew Ogilvy, Method of manufacture of a thixotropic deposit ,(1987)
Sergio Gallo, Merton C. Flemings, Hot chamber die casting of semisolids ,(2002)
Eiji Suzuki, Satoshi Terada, Hiroshi Ueda, Tetsuo Fujita, Tomoaki Komatsu, Shinichi Takayama, John C. Reed, Establishing apoptosis resistant cell lines for improving protein productivity of cell culture Cytotechnology. ,vol. 23, pp. 55- 59 ,(1997) , 10.1023/A:1007942929800