Normal cellular counterparts of B cell chronic lymphocytic leukemia.

摘要: In an attempt to compare B cell chronic lymphocytic leukemia (B-CLL) with its normal cellular counterpart, the surface phenotype of 100 cases B-CLL was determined by using a panel monoclonal antibodies (MoAbs) directed against cell-restricted and -associated antigens. The majority cells expressed Ia, B4 (CD19), B1 (CD20), B2 (CD21), immunoglobulin (sIg), T1 (CD5) but lacked C3b (CD35) receptors. contrast, overwhelming small unstimulated B4, B1, B2, sIg, receptors detectable T1. Small numbers weakly sIg+ could be identified in peripheral blood tonsil that coexpressed Approximately 16% fetal splenocytes T1, weak Ia therefore closely resembled most cells. With phenotypic differences between cells, we examined vitro activated for expression activation Of 20 examined, virtually all B5, approximately 50% interleukin-2 (IL-2R) Blast-1. Normal were either anti-Ig or 12-0-tetradecanoylphorbol-beta-acetate (TPA) then coexpression antigens B5 IL-2R. Only TPA as well proliferated presence IL-2, whereas did not, although they identical 60-kilodalton proteins immunoprecipitation. These studies are consistent notion resembles several minor subpopulations including population directly through protein kinase C pathway.