Biodegradation of acrylamide and purification of acrylamidase from newly isolated bacterium Moraxella osloensis MSU11
作者： S. Emmanuel Joshua Jebasingh , M. Lakshmikandan , R.P. Rajesh , P. Raja
DOI: 10.1016/J.IBIOD.2013.06.012
关键词: Pectinase 、 Moraxella osloensis 、 Acrylamide 、 Bacteria 、 Polyacrylamide 、 Chromatography 、 Chemistry 、 Effluent 、 Biodegradation 、 Environmental pollution
摘要: The increasing industrial utilization of polyacrylamide to assist water clarification, sludge conditioning, papermaking, and secondary oil recovery leads environmental pollution. In this work, an acrylamide degrading bacterium was isolated from paper mill effluent at Charan mahadevi, Tamilnadu, India. minimal medium containing (40 mM) served as a sole source carbon nitrogen for bacteria. bacterial strain has grown well in 40 mM pH (6-7) 30 degrees C. Within 24-48 h converted into acrylic acid other metabolites. Based on biochemical characteristics 16S rRNA gene sequence, the identified Gram negative, diplobacilli Moraxella osloensis MSU11. hydrolyzing enzyme acrylamidase purified by HPLC. molecular weight determined be approximately 38 kDa SDS-PAGE using reference Pectinase. These results show that M. MSU11 potential degrade present environment. (C) 2013 Elsevier Ltd. All rights reserved.
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