Comparison of four molecular markers for genetic analysis in Diospyros L. (Ebenaceae).
作者： X. Y. Du , Q. L. Zhang , Zheng-Rong Luo
DOI: 10.1007/S00606-009-0199-Z
关键词: Genotype 、 Genetics 、 Phylogenetic tree 、 Genetic marker 、 Genetic analysis 、 Genetic relationship 、 Polymorphism (computer science) 、 Biology 、 Amplified fragment length polymorphism 、 Molecular marker
摘要: Four molecular markers, including inter-retrotransposon amplified polymorphism (IRAP), retrotransposon-microsatellite (REMAP), sequence-specific (SSAP), and fragment length (AFLP), were compared in terms of their informativeness efficiency for analysis genetic relationships among 28 genotypes the genus Diospyros. The results as follows: (1) highest level detected observed IRAP; (2) AFLP was most efficient marker system due to simultaneous detection abundant markers per single reaction; (3) index (MI) value lower SSAP than AFLP, but had a higher AFLP; (4) correlation coefficients similarity statistically significant all four systems; (5) yielded similar phylogenetic trees. To our knowledge, this first detailed report comparison performance three retrotransposon-based (IRAP, REMAP, SSAP) technique (DNA-based marker) on set samples provide guidance future use these methods
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