A quick, simple and unbiased method to quantify C2C12 myogenic differentiation

摘要: Introduction: C2C12 myoblasts undergo in vitro myogenesis to form protein-rich multinucleated myotubes. Determining the fraction of total nuclei incorporated into myo- tubes is a commonly used method quantify extent dif- ferentiation, but it labor-intensive and susceptible operator bias. Methods: We have developed simple myotube formation using micrographs Jenner-Giemsa- stained cultures. Because myotubes are darkly by Jenner-Giemsa dyes, corre- lates with an increase pixels attributed darkest tones. Thus, image histograms were obtained from photographs ImageJ software, sum tones was as measure density. Results: Measurements density mirrored those fusion index during differentiation after treatment prostaglandin D2, in- hibitor myogenesis. Conclusions: propose this inexpensive, quick, unbiased ferentiation complement analysis. Muscle Nerve 44: 366-370, 2011