Production and characterization of poly(I):poly(C)-induced rat interferons in vitro.
作者： DANNY L. WIEDBRAUK , GARY R. BURLESON
关键词: Heterologous 、 Interferon 、 Neutralization 、 In vitro 、 Antiserum 、 Fibroblast 、 Biology 、 Molecular biology 、 Gel electrophoresis 、 Interferon inducer
摘要: The synthetic polyribonucleotide polyinosinate:polycytidylate [poly(I):poly(C)] was used to induce interferon (IFN) production in a rat leiomyosarcoma cell line. Three separate IFN species were distinguished by antiviral activity on homologous and heterologous cells, interaction with Blue Sepharose CL-6B chromatography matrix, antibody neutralization studies. Two species, one weakly ionic the other hydrophobic Sepharose, exhibited activity, acid- heat-labile. Both had molecular weight of 30,000 daltons HPLC-based exclusion chromatography. neutralized only antiserum L-cell IFN, whereas human leukocyte IFN. remaining heat-stable, no not any antisera This 39,000 34,000 sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These fibroblast IFNs possess greater heterogeneity than that reported or observed for murine IFNs. could thus be classified as follows: IFN-beta two different unique acid-labile
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