Purification of L cell interferon.

摘要: Publisher Summary This chapter describes various processes used for concentrating and purifying L cell interferon, starting from the crude material that consists of serum-free culture fluid Newcastle disease virus (NDV)-infected cells. In final part, a simple procedure is described to purify radiolabeled interferon (biosynthetic label) by immune precipitation. For successful purification, it essential start with large amount in terms biological activity. mainly two reasons, both related very high specific activity pure interferon: (a) keep protein concentration samples enough during purification avoid loss activity, which rapidly takes place dilute solutions (b) make possible monitor progress analyze purity preparations conventional methods detecting protein.