Photodynamic inhibition of acetylcholinesterase after two-photon excitation of copper tetrasulfophthalocyanine.
作者： Youssef Mir , Daniel Houde , Johan E. van Lier
DOI: 10.1007/S10103-007-0446-X
关键词: Irradiation 、 Enzyme assay 、 Acetylcholinesterase 、 Photochemistry 、 Chemistry 、 Reactive oxygen species 、 Photosensitizer 、 Copper 、 Photodynamic therapy 、 Two-photon excitation microscopy
摘要: Sequential two-photon (2-γ) activated copper tetrasulfophthalocyanine (CuPcS4) was shown capable of inactivating acetylcholinesterase (ACE). ACE activity measured photometrically by the Ellman method. Simultaneous irradiation in presence CuPcS4 with 514 nm (183 mW/cm2) and 670 (86 continuous wave (CW) light induced a 20–50% increase enzyme inhibition as compared to one-photon (1-γ) irradiation, using either 514- or 670-nm at same fluences. The not affected alone, decreased linearly time, be oxygen-dependent. We conclude that photoinactivation sequential 2-γ involves reactive oxygen species produced interaction upper excited Tn state molecular oxygen. As shows little conventional 1-γ photosensitizer, unwanted side effects such prolonged skin sensitivity are eliminated rendering photodynamic therapy advantageous for treatment selected medical applications.
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