Evaluation of the bioeffects of prenatal ultrasound exposure in the cynomolgus macaque (Macaca fascicularis): I. Neonatal/infant observations

摘要: The frequency of use ultrasonography for evaluating the developing embryo/fetus has continued to rise although possible risks from exposure still remain uncertain. cynomolgus macaque (Macaca fascicularis) is currently being used in our laboratory as a model assess these risks. In utero was performed utilizing commercial real-time mechanical sector scanner with 7.5 MHz scanhead (ATL, MK 600). Maximum acoustic power output this unit follows: I(SPTA) = 12.0 mW/cm2, I(SPPA) 98 W/cm2, and Im 137 W/cm2. Animals exposed ultrasound (N 16) were scanned five times weekly on gestational days (GD) 21-35 +/- 2 10 minutes/exam (m/e), three GD 36-60 m/e, once 61-150 20 m/e. Controls 14) "scanned" placed standby. Assessment simian Apgar scores at 1, 5, minutes life revealed higher treated animals (P less than or equal 0.045); greater muscle tone 0.013) color 0.016) observed. Evaluation morphometrics birth including weight, biparietal diameter, occipitofrontal head circumference, hand foot lengths, humerus femur arm chest tail length, skinfold thickness, crown-rump length (CRL) indicated significant reduction only two parameters, weight 0.027) CRL 0.033). Hematologic analysis 9 16 1 difference white blood cell counts (WBCs). Treated displayed lower WBCs reductions numbers segmented neutrophils monocytes all ages differences not by 5-6 months age. No abortions, gross malformations, stillbirths observed animals.