[6] Tandem mass spectrometry: Multisector magnetic instruments

摘要: Publisher Summary This chapter discusses tandem mass spectrometry. Tandem magnetic sector spectrometry was originally developed for investigations of ion chemistry and ion-molecule reaction dynamics. In the mid 1970s, its application to chemical analysis realized particularly complex mixtures. However, as evolved, it became clear that major in determining structure biomolecules truly mixture better accomplished by incorporating some kind chromatography analytical protocol. Its utility is not be negated because many isolates biomolecules, even after chromatographic separation, are Thus, determination or identification analyte requires both separation analysis, which can with this method. has gained acceptance peptide sequences, especially high structural diversity peptides, their importance biochemistry, limitations other more classical methods (e.g., Edman degradation).