PNA probes, probe sets, methods and kits pertaining to the detection of microorganisms
作者： Jens J. Hyldig-Nielsen , James M. Coull
DOI:
关键词: Pseudomonas fluorescens 、 Staphylococcus aureus 、 Salmonella 、 Bacillus (shape) 、 Multiplex 、 Bacteria 、 Microbiology 、 Biology 、 Pseudomonas aeruginosa 、 Pseudomonas
摘要: This invention is related to novel PNA probes, probe sets, methods and kits pertaining the detection of microorganisms. The this can be used detect, identify or quantitate one more organisms in a sample wherein are selected from group consisting E. coli, Staphylococcus aureus, Pseudomonas aeruginosa, cepatia, fluorescens bacterial genus including Salmonella genus, Bacillus genus. preferred probing nucleobase sequence probes detect bacteria listed above TCA-ATG-AGC-AAA-GGT ( coli ); GCT-TCT-CGT-CCG-TTC aureus CTG-AAT-CCA-GGA-GCA AAC-TTG-CTG-AAC-CAC aeruginosa CCA-TCG-CAT-CTA-ACA cepatia TCT-AGT-CAG-TCA-GTT CCG-ACT-TGA-CAG-ACC CCT-GCC-AGT-TTC-GAA (Salmonella genus); CTT-TGT-TCT-GTC-CAT (Bacillus GCT-GGC-CTA-GCC-TTC, GTC-CTC-CTT-GCG-GTT TTC-TCA-TCC-GCT-CGA (Pseudomonas genus). particularly well suited for use multiplex PNA-FISH assays.
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