Rapid detection, identification, and enumeration of Pseudomonas aeruginosa in bottled water using peptide nucleic acid probes.

摘要: A new chemiluminescent in situ hybridization (CISH) method that provides simultaneous detection, identification, and enumeration of Pseudomonas aeruginosa bottled water within 1 working day has been developed. Individual micro-colonies P. were detected directly on membrane filters following 5 h growth by use soybean peroxidase-labeled peptide nucleic acid (PNA) probes targeted to a species-specific sequence rRNA. Within each micro-colony, reaction the peroxidase with substrate generated light was subsequently captured film or digital camera system. Each spot represented one micro-colony aeruginosa. Sensitivity specificity for identification 100% as determined testing 28 strains 17 other bacterial species included closely related species. Furthermore, number spots correlated counts visible colonies sustained growth. We conclude PNA CISH speeds up traditional filtration techniques adds probe technology generate fast definitive results.