Expression of p53 and 17p allelic loss in colorectal carcinoma.

摘要: Mutations in the p53 gene are most common genetic changes cancer thus far. Many mutations result a protein product having prolonged half-life compared to wild-type p53. The mutant is frequently detectable immunohistochemically, whereas present normal cells not. We examined 90 colorectal carcinomas for increased expression of using 3 specific monoclonal antibodies, PAb1801, PAb421, and PAb240. Overall, 70% stained Each tumor's DNA was also assessed loss heterozygosity on chromosome 17p, location gene. Of those tumors that reacted with anti-p53 76% showed 17p. Tumors 17p generally all without tended stain just one antibody, typically Fifteen were presence mutations. A total 10 found, 6 missense 2 deletions, but