Time of Enterotoxin Formation and Release During Sporulation of Clostridium perfringens Type A

作者: Charles L. Duncan

DOI: 10.1128/JB.113.2.932-936.1973

关键词: EnterotoxinSporangiumIntracellularAntigenClostridium perfringensBiologyLysisToxinMicrobiologySporeMolecular biology

摘要: Clostridium perfringens enterotoxin was detected intracellularly about 3 hr after the inoculation of vegetative cells into sporulation medium. The subsequent increase in intracellular concentration roughly paralleled but followed by 2.5 to 5 number heat-resistant spores. biologically active toxin coincided with antigen. Enterotoxin released from sporangium its lysis, concomitantly mature spore release.

参考文章(14)
Charles L. Duncan, Ronald G. Labbe, Robert R. Reich, Germination of Heat- and Alkali-Altered Spores of Clostridium perfringens Type A by Lysozyme and an Initiation Protein Journal of Bacteriology. ,vol. 109, pp. 550- 559 ,(1972) , 10.1128/JB.109.2.550-559.1972
Charles L. Duncan, Eileen B. Somers, Quantitation of Clostridium perfringens Type A Enterotoxin by Electroimmunodiffusion Applied Microbiology. ,vol. 24, pp. 801- 804 ,(1972) , 10.1128/AM.24.5.801-804.1972
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
Carol A. Miller, Arthur W. Anderson, Rapid Detection and Quantitative Estimation of Type A Botulinum Toxin by Electroimmunodiffusion Infection and Immunity. ,vol. 4, pp. 126- 129 ,(1971) , 10.1128/IAI.4.2.126-129.1971
P Schaeffer, Sporulation and the production of antibiotics, exoenzymes, and exotonins. Bacteriological Reviews. ,vol. 33, pp. 48- 71 ,(1969) , 10.1128/BR.33.1.48-71.1969
A. C. Emeruwa, R. Z. Hawirko, Comparative studies of an asporogenic mutant and a wild type strain of Clostridium botulinum type E Canadian Journal of Microbiology. ,vol. 18, pp. 29- 34 ,(1972) , 10.1139/M72-005
A. H. W. Hauschild, Erythemal activity of the cellular enteropathogenic factor of Clostridium perfringens type A. Canadian Journal of Microbiology. ,vol. 16, pp. 651- 654 ,(1970) , 10.1139/M70-112
Manuel Lopez, Theta Tsu, Newton E. Hyslop, Studies of electroimmunodiffusion: Immunochemical quantitation of proteins in dilute solutions Immunochemistry. ,vol. 6, pp. 513- 526 ,(1969) , 10.1016/0019-2791(69)90191-8