Expression of Signal Transduction System Encoding Genes of Yersinia pseudotuberculosis IP32953 at 28°C and 3°C
作者： Eveliina Palonen , Miia Lindström , Reija Karttunen , Panu Somervuo , Hannu Korkeala
DOI: 10.1371/JOURNAL.PONE.0025063
关键词: Genetics 、 Signal transduction 、 rpoS 、 Gene expression 、 Gene expression profiling 、 Gene 、 Phenotype 、 Yersinia pseudotuberculosis 、 Biology 、 Regulator 、 General Biochemistry, Genetics and Molecular Biology 、 General Agricultural and Biological Sciences 、 General Medicine
摘要: Yersinia pseudotuberculosis is a significant psychrotrophic food pathogen whose cold tolerance mechanisms are poorly understood. Signal transduction systems serve to monitor the environment, but no systematic investigation of their role at temperatures in Y. has yet been undertaken. The relative expression levels 54 genes predicted encode proteins belonging signal IP32953 were determined 28°C and 3°C by quantitative real-time reverse transcription-PCR. 44 significantly (p<0.05) higher than 28°C. Genes encoding two-component system CheA/CheY had highest 3°C. Mutational analysis revealed that cheA important for growth motility level one gene, rssB, an RpoS regulator, was lower results suggest several might be used during low temperature, least, low-temperature growth.
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