Deep metagenomics examines the oral microbiome during dental caries, revealing novel taxa and co-occurrences with host molecules.

摘要: Dental caries, the most common chronic infectious disease worldwide, has a complex etiology involving interplay of microbial and host factors that are not completely understood. In this study, oral microbiome 38 cytokines chemokines were analyzed across 23 children with caries 24 healthy dentition. De novo assembly metagenomic sequencing obtained 527 metagenome-assembled genomes (MAGs), representing 150 bacterial species. Forty-two these species had no in public repositories, thereby novel taxa. These new greatly expanded known pangenomes many clades, including enigmatic Saccharibacteria clades G3 G6, which distinct functional repertoires compared to other Saccharibacteria. understood be obligate epibionts, dependent on bacteria. data suggest various may rely their hosts for highly metabolic requirements, would have significant evolutionary ecological implications. Across study group, Rothia, Neisseria, Haemophilus spp. associated good dental health, whereas Prevotella spp., Streptococcus mutans, Human herpesvirus 4 (Epstein-Barr virus [EBV]) more prevalent caries. Finally, 10 immunological markers significantly elevated co-occurrence analysis provided an atlas potential relationships between microbes molecules. Overall, illustrated at unprecedented resolution contributed several leads further will increase understanding pathogenesis guide therapeutic development.