Dystrophin Dp71 is critical for stability of the DAPs in the nucleus of PC12 cells.
作者： Marcela Villarreal-Silva , Rocío Suárez-Sánchez , Rafael Rodríguez-Muñoz , Dominique Mornet , Bulmaro Cisneros
DOI: 10.1007/S11064-009-0064-Z
关键词: Gene product 、 Immunoprecipitation 、 PC12 cell line 、 Cytoplasm 、 Gene isoform 、 Nuclear matrix 、 Biology 、 Molecular biology 、 Utrophin 、 Nucleus
摘要: We have adopted the PC12 cell line as in vitro model for studying Dp71 function neuronal cells. These cells express a cytoplasmic (Dp71f) and nuclear (Dp71d) isoform of well various dystrophin-associated proteins (DAPs). In this study, we revealed by confocal microscopy analysis Western blotting evaluation fractions presence different DAPs (β-dystroglycan, β-dystrobrevin, e-sarcoglycan γ1-syntrophin) nucleus Furthermore, established immunoprecipitation assays that Dp71d form protein complex (DAPC) nucleus. Interestingly, depletion antisense treatment (antisense-Dp71 cells) provoked drastic reduction DAPs, which indicates is critical stability within Although Up71, utrophin gene product homologous to Dp71, exhibited increased expression antisense-Dp71 cells, its scarce levels makes unlikely could compensate deficiency.
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