Restriction Endonuclease-Mediated Selective Polymerase Chain Reaction
作者： Robyn Ward , Nicholas Hawkins , Roslynn O'Grady , Catherine Sheehan , Terence O'Connor
DOI: 10.1016/S0002-9440(10)65581-2
关键词: Taq polymerase 、 Restriction enzyme 、 Biology 、 DNA 、 Gel electrophoresis 、 Gene mutation 、 Point mutation 、 Polymerase chain reaction 、 Mutation 、 Molecular biology
摘要: … Mutations at the first and second bases of codon 12 of the … for 2 minutes (one cycle) and 58C for 60 seconds followed by … PCR amplification.29, 30 Our study indicated that, in the case of …
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