Human α-Fetoprotein

摘要: Abstract Human α-fetoprotein has been isolated from the sera of hepatoma patients by combined techniques preparative electrophoresis, Sephadex gel filtration, and isoelectric focusing. The purity was demonstrable (a) a single line on Ouchterlony immunodiffusion versus anti-α-fetoprotein, with no reaction potent polyvalent anti-human serum anti-albumin; (b) band sodium dodecyl sulfate polyacrylamide electrophoresis; (c) production monospecific antibody upon immunization rabbits. molecular weight 72,000 acrylamide electrophoresis. Two major forms were found focusing, ion exchange chromatography, agarose Both present in most fetal sera, but their relative amounts appeared to have relationship gestational age, clinical status, concentration, or method storage. two forms, points 4.85 5.2, focusing convert homogeneous form slower electrophoretic mobility after treatment neuraminidase.