MicroRNA-137 promoter methylation as an etiologic and prognostic biomarker for squamous cell carcinoma of the head and neck

摘要: Head and neck cancer accounted for 3.3% of incident malignancies 2.0% cancer-deaths in the US 2009, majority which are squamous origin. Thus, there is a need novel biomarkers early detection prognosis cell carcinoma head (SCCHN). MicroRNA-137 plays role cycle control through negative regulation Cdk6, has been reported to undergo promoter methylation oral carcinoma. Oral rinse non-invasive mode DNA collection, may have some utility methylation. The primary goals this research were determine if miR-137 occurs all SCCHN, including pharyngeal laryngeal tumors, whether it detectable samples; assess as an etiologic prognostic biomarker SCCHN. was extracted from rinses 99 SCCHN patients cancer-free subjects tumor tissue 67 patients; paired available 64 patients. Promoter status determined by methylation-specific PCR. We identified strong association between detected (OR = 4.80, 95% CI: 1.23-18.82). There positive female gender 5.30, 1.20-23.44) inverse with body mass index 0.88, 0.77-0.99). associated poorer overall survival (HR 3.68, 1.01-13.38). In spite its low sensitivity (21.2%), future value panels diagnosis due high specificity (97.0%) occurrence stage disease; promise marker. identification diagnostic such will significantly impact public health reduction morbidity mortality that result disease.