Active Suppression of Interneuron Programs within Developing Motor Neurons Revealed by Analysis of Homeodomain Factor HB9
作者： Joshua Thaler , Kathleen Harrison , Kamal Sharma , Karen Lettieri , John Kehrl
DOI: 10.1016/S0896-6273(01)80027-1
关键词: Downregulation and upregulation 、 In situ hybridization 、 Gene expression 、 Homeobox 、 Transcription factor 、 Biology 、 Neuroscience 、 Interneuron 、 Cellular differentiation 、 Sonic hedgehog 、 General Neuroscience
摘要: Sonic hedgehog (Shh) specifies the identity of both motor neurons (MNs) and interneurons with morphogen-like activity. Here, we present evidence that homeodomain factor HB9 is critical for distinguishing MN interneuron in mouse. Presumptive progenitors postmitotic MNs express HB9, whereas never this factor. This pattern resembles a composite avian homologs MNR2 HB9. In mice lacking Hb9, genetic profile significantly altered, particularly by upregulation Chx10, gene normally restricted to class ventral interneurons. aberrant expression accompanied topological disorganization columns, loss phrenic abducens nerves, intercostal nerve pathfinding defects. Thus, actively suppress programs establish their identity.
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