Assessment of the antibacterial activity of tea tree oil using the European EN 1276 and EN 12054 standard suspension tests

作者: S. Messager , K.A. Hammer , C.F. Carson , T.V. Riley

DOI: 10.1016/J.JHIN.2004.07.015

关键词: Tea tree oilStaphylococcus aureusAntibacterial activityPseudomonadaceaeMicrobiologyMicrococcaceaeAntibacterial agentBiologyAcinetobacter baumanniiPseudomonas aeruginosa

摘要: The activity of tea tree oil (TTO) and TTO-containing products was investigated according to the EN 1276 12054 European suspension methods. different concentrations TTO, a hygienic skin wash (HSW), an alcoholic (AHSW) hand rub (AHR) investigated. These formulations were assessed in perfect conditions with test, as well presence interfering substances against Staphylococcus aureus, Acinetobacter baumannii, Escherichia coli Pseudomonas aeruginosa. With latter same without TTO also control. AHR achieved >10(5)-fold reduction all four test organisms within 1-min contact time. AHSW >or=10(5)-fold A. baumannii after time S. E. P. aeruginosa 5-min efficacy appeared be dependent on formulation concentration tested, and, lastly, organism tested. Nevertheless, 5% >10(4)-fold cell numbers conditions. (5%) 0.001% Tween 80 significantly more active than aureus baumannii. time, numbers, respectively, >4 log10 used this study are now being tested using novel ex vivo method standard handwashing 1499.

参考文章(42)
S.E. Walsh, J.-Y. Maillard, A.D. Russell, C.E. Catrenich, D.L. Charbonneau, R.G. Bartolo, Activity and mechanisms of action of selected biocidal agents on Gram-positive and -negative bacteria. Journal of Applied Microbiology. ,vol. 94, pp. 240- 247 ,(2003) , 10.1046/J.1365-2672.2003.01825.X
Katherine A. Hammer, Christine F. Carson, Thomas V. Riley, Susceptibility of transient and commensal skin flora to the essential oil of Melaleuca alternifolia (tea tree oil). American Journal of Infection Control. ,vol. 24, pp. 186- 189 ,(1996) , 10.1016/S0196-6553(96)90011-5
David Herrera, Silvia Roldán, Isabel Santacruz, Sagrario Santos, Mireia Masdevall, Mariano Sanz, Differences in antimicrobial activity of four commercial 0.12% chlorhexidine mouthrinse formulations: an in vitro contact test and salivary bacterial counts study Journal of Clinical Periodontology. ,vol. 30, pp. 307- 314 ,(2003) , 10.1034/J.1600-051X.2003.00341.X
Christine F. Carson, Thomas V. Riley, Toxicity of the essential oil of Melaleuca alternifolia or tea tree oil Clinical Toxicology. ,vol. 33, pp. 193- 194 ,(1995) , 10.3109/15563659509000474
K. A. Hammer, L. Dry, M. Johnson, E. M. Michalak, C. F. Carson, T. V. Riley, Susceptibility of oral bacteria to Melaleuca alternifolia (tea tree) oil in vitro Oral Microbiology and Immunology. ,vol. 18, pp. 389- 392 ,(2003) , 10.1046/J.0902-0055.2003.00105.X
S.D. Cox, C.M. Mann, J.L. Markham, Interactions between components of the essential oil of Melaleuca alternifolia. Journal of Applied Microbiology. ,vol. 91, pp. 492- 497 ,(2001) , 10.1046/J.1365-2672.2001.01406.X
Marcio Guilhermetti, Silvio Evandro Daniel Hernandes, Yoshiaki Fukushigue, Lourdes Botelho Garcia, Celso Luíz Cardoso, Effectiveness of hand-cleansing agents for removing methicillin-resistant Staphylococcus aureus from contaminated hands. Infection Control and Hospital Epidemiology. ,vol. 22, pp. 105- 108 ,(2001) , 10.1086/501872
Kazue Nishioka, Tokuji Seguchi, Hidetoshi Yasuno, Toshihiko Yamamoto, Kazuyuki Tominaga, The results of ingredient patch testing in contact dermatitis elicited by povidone-iodine preparations. Contact Dermatitis. ,vol. 42, pp. 90- 94 ,(2000) , 10.1034/J.1600-0536.2000.042002090.X
C. Brand, A. Ferrante, R.H. Prager, T.V. Riley, C.F. Carson, J.J. Finlay-Jones, P.H. Hart, The water-soluble components of the essential oil of Melaleuca alternifolia (tea tree oil) suppress the production of superoxide by human monocytes, but not neutrophils, activated in vitro. Inflammation Research. ,vol. 50, pp. 213- 219 ,(2001) , 10.1007/S000110050746