The biochemical basis of nitroblue tetrazolium reduction in normal human and chronic granulomatous disease polymorphonuclear leukocytes

摘要: Normal human polymorphonuclear leukocytes (PMN) placed in anaerobic chambers reaching pO2's of less than 5 mm Hg fail to generate O2-, iodinate ingested particles, and stimulate glucose-1-14C oxidation through the hexose monophosphate shunt. The observation that cells are incapable generating O2- or reducing nitroblue tetrazolium (NBT) formazan supports idea NBT reduction phagocytizing PMN is due exclusively oxygen-dependent oxidase which deficient chronic granulomatous disease leukocytes, despite their hyperphagocytic capacity.