The sequence of a male-specific genome region containing the sex determination switch in Aedes aegypti

摘要: Aedes aegypti is the principal vector of several important arboviruses. Among methods control to limit transmission disease are genetic strategies that involve release sterile or genetically modified non-biting males, which has generated interest in manipulating mosquito sex ratios. Sex determination Ae. controlled by a non-recombining Y chromosome-like region called M locus, yet characterisation this locus been thwarted repetitive nature genome. In 2015, an gene named Nix was identified displays qualities switch. With use whole-genome bacterial artificial chromosome (BAC) library, we amplified and sequenced ~200 kb containing male-determining Nix. study, show comprised two exons separated 99 intron primarily composed DNA, especially transposable elements. Nix, unusually large highly gene, exhibits features common with genes other organisms. We speculate lack recombination at allowed expansion repeats manner characteristic sex-limited chromosome, accordance proposed models evolution insects.