Mutagen-induced changes in cellular deoxycytidine triphosphate and thymidine triphosphate in Chinese hamster ovary cells
作者： C.N. Newman , J.H. Miller
DOI: 10.1016/0006-291X(83)91590-5
关键词: DNA synthesis 、 DNA replication 、 Chinese hamster ovary cell 、 Cell killing 、 Deoxycytidine triphosphate 、 Thymidine triphosphate 、 Biology 、 Mutagen 、 Thymidine 、 Molecular biology 、 Biochemistry
摘要: Abstract Deoxynucleoside triphosphate concentrations in Chinese hamster ovary cell lines, CHO-K1 and Mut 8–16, were examined following exposure of cells to UV or dimethylsulfate. Marked decreases dCTP observed 2 hr after both mutagens. In contrast, dTTP increased with killing but not Examination DNA synthesis permeabilized the presence excess dNTP substrates suggests that enhances replication while inhibits replication. We therefore ask whether increase ration mutagenized whole either contributes prolongs induced inhibition addition we proposed such an imbalance may also contribute mutations by enhancing probability for base-misincorporation.
elsevier.com
sci-hub.se 参考文章(17)
T A Kunkel, L A Loeb, On the fidelity of DNA replication. Effect of divalent metal ion activators and deoxyrionucleoside triphosphate pools on in vitro mutagenesis. Journal of Biological Chemistry. ,vol. 254, pp. 5718- 5725 ,(1979) , 10.1016/S0021-9258(18)50474-9
T.A. Kunkel, R.M. Schaaper, R.A. Beckman, L.A. Loeb, On the fidelity of DNA replication. Effect of the next nucleotide on proofreading. Journal of Biological Chemistry. ,vol. 256, pp. 9883- 9889 ,(1981) , 10.1016/S0021-9258(19)68712-0
Detection of activities that interfere with the enzymatic assay of deoxyribonucleoside 5'-triphosphates. Journal of Biological Chemistry. ,vol. 255, pp. 6640- 6645 ,(1980) , 10.1016/S0021-9258(18)43617-4
C R Ashman, R L Davidson, Bromodeoxyuridine mutagenesis in mammalian cells is related to deoxyribonucleotide pool imbalance. Molecular and Cellular Biology. ,vol. 1, pp. 254- 260 ,(1981) , 10.1128/MCB.1.3.254
C.N. Newman, Characterization of a UV-resistant mutant of CHO-K1 with normal repair activity. Mutation Research. ,vol. 96, pp. 99- 108 ,(1982) , 10.1016/0027-5107(82)90020-3
Mark MEUTH, Eva AUFREITER, Peter REICHARD, Deoxyribonucleotide pools in mouse-fibroblast cell lines with altered ribonucleotide reductase. FEBS Journal. ,vol. 71, pp. 39- 43 ,(1976) , 10.1111/J.1432-1033.1976.TB11087.X
Nathan A. Berger, Aaron S. Kaichi, Palmer G. Steward, Robert R. Klevecz, Gerald L. Forrest, Stephen D. Gross, Synthesis of poly(adenosine diphosphate ribose) in synchronized Chinese hamster cells. Experimental Cell Research. ,vol. 117, pp. 127- 135 ,(1978) , 10.1016/0014-4827(78)90435-4
J.E. Cleaver, G.H. Thomas, S.D. Park, Xeroderma pigmentosum variants have a slow recovery of DNA synthesis after irradiation with ultraviolet light Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis. ,vol. 564, pp. 122- 131 ,(1979) , 10.1016/0005-2787(79)90193-X
Dan Dahle, T. D. Griffiths, J. G. Carpenter, INHIBITION AND RECOVERY OF DNA SYNTHESIS IN UV‐IRRADIATED CHINESE HAMSTER V‐79 CELLS† Photochemistry and Photobiology. ,vol. 32, pp. 157- 165 ,(1980) , 10.1111/J.1751-1097.1980.TB04003.X
Mark Meuth, Role of deoxynucleoside triphosphate pools in the cytotoxic and mutagenic effects of DNA alkylating agents. Somatic Cell and Molecular Genetics. ,vol. 7, pp. 89- 102 ,(1981) , 10.1007/BF01544750