Deoxyribonucleoside triphosphate imbalance. 5-Fluorodeoxyuridine-induced DNA double strand breaks in mouse FM3A cells and the mechanism of cell death.
作者： A Yoshioka , S Tanaka , O Hiraoka , Y Koyama , Y Hirota
DOI: 10.1016/S0021-9258(18)47554-0
关键词:
摘要: The mechanism of cytotoxic action 5-fluorodeoxyuridine (FdUrd) in mouse FM3A cells was investigated. We observed the FdUrd-induced imbalance intracellular deoxyribonucleoside triphosphate (dNTP) pools and subsequent double strand breaks mature DNA, accompanied by cell death. dNTP maximal at 8 h after 1 microM FdUrd treatment; a depletion dTTP dGTP an increase dATP pool were observed. addition culture medium induced giving rise to 90 S peak alkaline sucrose gradient sedimentation. loss viability colony-forming ability occurred about 10 h. DNA as measured neutral elution method also FdUrd-treated addition. These results lead us propose that play important role FdUrd-mediated A comparison ratio single following radiation suggested produced exclusively. Cycloheximide inhibited both production An activity can induce detected lysate but not untreated cells. This suggests induces cellular breaking activity. death appear occur phase. Our indicate is trigger for break
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