Platelet size does not correlate with platelet age
作者： CB Thompson , DG Love , PG Quinn , CR Valeri
DOI: 10.1182/BLOOD.V62.2.487.487
关键词: Biochemistry 、 Platelet 、 Endocrinology 、 Bone marrow 、 Baboon 、 Centrifugation 、 Isotopes of chromium 、 In vivo 、 Mean platelet volume 、 Lactate dehydrogenase 、 Chemistry 、 Internal medicine
摘要: The relationship between platelet size and in vivo aging was investigated the baboon using size-dependent subpopulations separated by counterflow centrifugation. separation characteristics, size, lactate dehydrogenase (LDH) activity, dense- body content of were similar to those previously observed studies human platelets. Three independent labeling techniques used: (1) with 75Se-methionine, (2) vitro 51Cr, (3) 14C-serotonin. Maximal incorporation all three labels showed a close correlation mean volume (MPV) each fraction radioactivity. onset rate accumulation 75Se-methionine comparable fractions when corrected for differences volume, suggesting that heterogeneity present from time release platelets bone marrow. Survival 51Cr 14C-serotonin no translocation label one another circulation over time. In survival values radionuclides slight but significant lifespan MPV fractions. data suggest large not younger platelets, rather longer life-span. Platelet is result production factors marrow maturation circulation.
ashpublications.org
osti.gov
bloodjournal.org参考文章(26)
L Corash, B Shafer, Use of asplenic rabbits to demonstrate that platelet age and density are related. Blood. ,vol. 60, pp. 166- 171 ,(1982) , 10.1182/BLOOD.V60.1.166.166
JM Paulus, Platelet size in man Blood. ,vol. 46, pp. 321- 336 ,(1975) , 10.1182/BLOOD.V46.3.321.321
J. David Bessman, Larry J. Williams, P. Ridgway Gilmer, Mean Platelet Volume. The Inverse Relation of Platelet Size and Count in Normal Subjects, and an Artifact of Other Particles American Journal of Clinical Pathology. ,vol. 76, pp. 289- 293 ,(1981) , 10.1093/AJCP/76.3.289
Simon Karpatkin, Arthur Charmatz, Heterogeneity of human Platelets Journal of Clinical Investigation. ,vol. 48, pp. 1073- 1082 ,(1969) , 10.1172/JCI106063
D Deykin, J A Jakubowski, C B Thompson, P G Quinn, C R Valeri, Platelet size as a determinant of platelet function Journal of Laboratory and Clinical Medicine. ,vol. 101, pp. 205- 213 ,(1983)
Morris A. Blajchman, Andrew F. Senyi, Jack Hirsh, Edward Genton, James N. George, Hemostatic Function, Survival, and Membrane Glycoprotein Changes in Young versus Old Rabbit Platelets Journal of Clinical Investigation. ,vol. 68, pp. 1289- 1294 ,(1981) , 10.1172/JCI110375
Joseph A. Jakubowski, Craig B. Thompson, Raymond Vaillancourt, C. Robert Valeri, Daniel Deykin, Arachidonic acid metabolism by platelets of differing size British Journal of Haematology. ,vol. 53, pp. 503- 511 ,(1983) , 10.1111/J.1365-2141.1983.TB02052.X
D. G. PENINGTON, N. L. Y. LEE, A. E. ROXBURGH, J. R. MCGREADY, Platelet density and size: the interpretation of heterogeneity. British Journal of Haematology. ,vol. 34, pp. 365- 376 ,(1976) , 10.1111/J.1365-2141.1976.TB03583.X
A.J. Melaragno, W. Abdu, R. Katchis, A. Doty, C.R. Valeri, Liquid and freeze preservation of baboon platelets. Cryobiology. ,vol. 18, pp. 445- 452 ,(1981) , 10.1016/0011-2240(81)90202-9
Stephen R. Hanson, Laurence A. Harker, Survival of baboon platelets labeled with diazotized (125-1)-iodosulfanilic acid: No effect of drugs that modify platelet behavior Thrombosis Research. ,vol. 23, pp. 133- 143 ,(1981) , 10.1016/0049-3848(81)90246-2