Characteristics Of Laccase Immobilized On Different Supports For Wine-Making Technology
作者： A. Lante , A. Crapisi , G. Pasini , A. Zamorani , P. Spettoli
DOI: 10.1016/S1569-2558(08)60311-2
关键词: Adsorption 、 Glutaraldehyde 、 Chemistry 、 Chromatography 、 Silica gel 、 Laccase 、 Polysaccharide 、 Colloidal silica 、 Polymerization 、 Wine
摘要: The results of a study commercial laccase immobilized by covalent binding on CH-Sepharose 4B, CNBr-Sepharose VA-Hydroxy, and VA-Epoxy carriers, adsorption followed treatment with glutaraldehyde silica gel Florisil, entrapment colloidal silica, radiation polymerization are reported here. polysaccharide carriers displayed the best activity yields ranging from 51 to 63%. By contrast, enzyme physical showed 100% immobilization yields. caused decrease in V max an increase K m factor 2.5 5.3 2.4 3.7, respectively. A phenolic model solution, both musts wine laccase, was also obtained.
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