A new immobilization technique of whole cells and enzymes with colloidal silica and alginate.
作者： Yaichi Fukushima , Katsutoshi Okamura , Kazutaka Imai , Hiroshi Motai
关键词:
摘要: A mixed gel composed of colloidal silica and alginate (As gel) was prepared for the immobilization enzymes or microorganisms. The physical strength AS increased with amount silica. ethanol production rate Saccharomyces cerevisiae (IFO 0224) immobilized in higher than (Al early phase growth. At a concentration glucose more 10%, yeast Al gel. Any difference not recognized diffusion coefficient between gels. had an ability to retain proteins such as bovine serum albumin gamma-globulin. alkaline protease beta-galactosidase continued their function long time, but those did not. Immobilized thermal stability free enzymes.
sci-hub.se 参考文章(8)
F. H. White, A. D. Portno, CONTINUOUS FERMENTATION BY IMMOBILIZED BREWERS YEAST Journal of the Institute of Brewing. ,vol. 84, pp. 228- 230 ,(1978) , 10.1002/J.2050-0416.1978.TB03878.X
O.V. Lomako, I.I. Menyailova, L.A. Nakhapetyan, Yu. Nikitin, A.V. Kiselev, Immobilization of glucoamylase on porous silicas Enzyme and Microbial Technology. ,vol. 4, pp. 89- 92 ,(1982) , 10.1016/0141-0229(82)90087-4
Joseph Bieth, Bernard Spiess, Camille G. Wermuth, The synthesis and analytical use of a highly sensitive and convenient substrate of elastase Biochemical Medicine. ,vol. 11, pp. 350- 357 ,(1974) , 10.1016/0006-2944(74)90134-3
Hideo Tanaka, Hiroshi Kurosawa, Etsuo Kokufuta, I. A. Veliky, Preparation of immobilized glucomylase using ca-alginate gel coated with partially quaterized poly(ethleneimine). Biotechnology and Bioengineering. ,vol. 26, pp. 1393- 1394 ,(1984) , 10.1002/BIT.260261118
Kazuhiro NAKANISHI, Shuji ADACHI, Shuichi YAMAMOTO, Ryuichi MATSUNO, Atsuo TANAKA, Tadashi KAMIKUBO, Fundamental studies on immobilized enzymes. II. Diffusion of saccharides and amino acids in cross-linked polymers. Agricultural and biological chemistry. ,vol. 41, pp. 2455- 2462 ,(1977) , 10.1271/BBB1961.41.2455
M. Kierstan, C. Bucke, The immobilization of microbial cells, subcellular organelles, and enzymes in calcium alginate gels Biotechnology and Bioengineering. ,vol. 19, pp. 387- 397 ,(2000) , 10.1002/BIT.260190309
Qayyum Husain, Jawaid Iqbal, M. Saleemuddin, Entrapment of concanavalin A‐glycoenzyme complexes in calcium alginate gels Biotechnology and Bioengineering. ,vol. 27, pp. 1102- 1107 ,(1985) , 10.1002/BIT.260270803
Zahid MOZAFFAR, Kazuhiro NAKANISHI, Ryuichi MATSUNO, Tadashi KAMIKUBO, Purification and Properties of β-Galactosidases from Bacillus circulans Agricultural and biological chemistry. ,vol. 48, pp. 3053- 3061 ,(1984) , 10.1271/BBB1961.48.3053