High expression of novel biomarker KRT16P3 promotes the progression of tongue squamous cell carcinoma and predicts poor prognosis.

作者: Yifan Yang , Ling Feng , Hongzhi Ma , Ru Wang , Jugao Fang

DOI: 10.1111/JOP.13138

关键词: Western blotApoptosisGentamicin protection assayCancer researchBiologyLong non-coding RNACellStat3 Signaling PathwayFlow cytometryGene knockdown

摘要: Background Overexpression of long non-coding RNAs (lncRNAs) reveals the abnormal pathological processes in many human cancers. KRT16P3, a novel overexpressed lncRNA tongue squamous cell carcinoma (TSCC), was identified by previous microarrays. However, role KRT16P3 TSCC is not clear. Methods Quantitative real-time polymerase chain reaction (qRT-PCR) used to detect expression tissues and cells. Next, relationships between clinical significance patients were analyzed. Additionally, Cell Counting Kit-8, 5-Bromo-2-deoxyuridine (BrdU) incorporation assay, colony formation flow cytometry apoptosis analysis, scratch wound healing transwell invasion assay explore biological function KRT16P3. Western blot qRT-PCR determine epithelial-mesenchymal transition (EMT) markers. The pathway changes after knockdown detected blot. Results We found significantly upregulated positively associated with advanced clinicopathological features patients, it may serve as poor prognostic factor. Functionally, inhibits proliferation, migration, promotes Furthermore, we also revealed that suppresses EMT JAK2/STAT3 signaling pathway. Conclusion Our results validated can modulate malignant progression, process, TSCC, which might biomarker an attractive target for patients.

参考文章(27)
Dan Grandér, Per Johnsson, Pseudogene-Expressed RNAs: Emerging Roles in Gene Regulation and Disease Current Topics in Microbiology and Immunology. ,vol. 394, pp. 111- 126 ,(2015) , 10.1007/82_2015_442
Laura Poliseno, Leonardo Salmena, Jiangwen Zhang, Brett Carver, William J. Haveman, Pier Paolo Pandolfi, A coding-independent function of gene and pseudogene mRNAs regulates tumour biology Nature. ,vol. 465, pp. 1033- 1038 ,(2010) , 10.1038/NATURE09144
P. P. Amaral, M. E. Dinger, T. R. Mercer, J. S. Mattick, The eukaryotic genome as an RNA machine Science. ,vol. 319, pp. 1787- 1789 ,(2008) , 10.1126/SCIENCE.1155472
P.C. Rodrigues, M.C.C. Miguel, E. Bagordakis, F.P. Fonseca, S.N. de Aquino, A.R. Santos-Silva, M.A. Lopes, E. Graner, T. Salo, L.P. Kowalski, R.D. Coletta, Clinicopathological prognostic factors of oral tongue squamous cell carcinoma: a retrospective study of 202 cases International Journal of Oral and Maxillofacial Surgery. ,vol. 43, pp. 795- 801 ,(2014) , 10.1016/J.IJOM.2014.01.014
, Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 99, pp. 16899- 16903 ,(2002) , 10.1073/PNAS.242603899
John J. O'Shea, Daniella M. Schwartz, Alejandro V. Villarino, Massimo Gadina, Iain B. McInnes, Arian Laurence, The JAK-STAT Pathway: Impact on Human Disease and Therapeutic Intervention* Annual Review of Medicine. ,vol. 66, pp. 311- 328 ,(2015) , 10.1146/ANNUREV-MED-051113-024537
Simon A. Joosse, Juliane Hannemann, Julia Spötter, Andreas Bauche, Antje Andreas, Volkmar Müller, Klaus Pantel, Changes in Keratin Expression during Metastatic Progression of Breast Cancer: Impact on the Detection of Circulating Tumor Cells Clinical Cancer Research. ,vol. 18, pp. 993- 1003 ,(2012) , 10.1158/1078-0432.CCR-11-2100
Julia Beermann, Maria-Teresa Piccoli, Janika Viereck, Thomas Thum, Non-coding RNAs in Development and Disease: Background, Mechanisms, and Therapeutic Approaches Physiological Reviews. ,vol. 96, pp. 1297- 1325 ,(2016) , 10.1152/PHYSREV.00041.2015