作者: Yifan Yang , Ling Feng , Hongzhi Ma , Ru Wang , Jugao Fang
DOI: 10.1111/JOP.13138
关键词: Western blot 、 Apoptosis 、 Gentamicin protection assay 、 Cancer research 、 Biology 、 Long non-coding RNA 、 Cell 、 Stat3 Signaling Pathway 、 Flow cytometry 、 Gene knockdown
摘要: Background Overexpression of long non-coding RNAs (lncRNAs) reveals the abnormal pathological processes in many human cancers. KRT16P3, a novel overexpressed lncRNA tongue squamous cell carcinoma (TSCC), was identified by previous microarrays. However, role KRT16P3 TSCC is not clear. Methods Quantitative real-time polymerase chain reaction (qRT-PCR) used to detect expression tissues and cells. Next, relationships between clinical significance patients were analyzed. Additionally, Cell Counting Kit-8, 5-Bromo-2-deoxyuridine (BrdU) incorporation assay, colony formation flow cytometry apoptosis analysis, scratch wound healing transwell invasion assay explore biological function KRT16P3. Western blot qRT-PCR determine epithelial-mesenchymal transition (EMT) markers. The pathway changes after knockdown detected blot. Results We found significantly upregulated positively associated with advanced clinicopathological features patients, it may serve as poor prognostic factor. Functionally, inhibits proliferation, migration, promotes Furthermore, we also revealed that suppresses EMT JAK2/STAT3 signaling pathway. Conclusion Our results validated can modulate malignant progression, process, TSCC, which might biomarker an attractive target for patients.