Raster image correlation spectroscopy as a novel tool to study interactions of macromolecules with nanofiber scaffolds.

摘要: Abstract Dynamic processes such as diffusion and binding/unbinding of macromolecules (e.g. growth factors or nutrients) are crucial parameters for the design application effective artificial tissue materials. Here, dynamics selected were studied in two different composite engineering scaffolds containing an electrospun nanofiber mesh (polycaprolactone hydrophobically plasma modified polyvinylalcohol–chitosan) encapsulated agarose hydrogels by a conventional approach fluorescence recovery after photobleaching (FRAP) novel technique, raster image correlation spectroscopy (RICS). The approaches compared, it is shown that FRAP unable to determine occurring at low molecular concentrations, especially accurately separating from diffusion, its results depend on concentration molecules. RICS measures single molecules and, because multiple adjustable timescales, can distinguish whether binding controls movement separates fast slow transient binding. In addition, provides robust read-out parameter quantifying affinity. Finally, combination helps characterize tested tissues better, therefore predicts behavior biologically active these materials medical applications.