Fas-ligand is stored in secretory lysosomes of ocular barrier epithelia and released with microvesicles.

摘要: Abstract Previously we described the release of hr44 from ciliary epithelium to coincide with loss late endosomal/lysosomal marker protein CD63 in mildly inflamed rat eyes. We showed that both proteins are released microvesicles into supernatant cultured retinal pigmented epithelial cells (ARPE-19). Here wish determine whether there is a concomitant fas-ligand (FasL) vivo and ocular have secretory lysosomes similar T cells, where FasL could derive. plays an important role immunity, immune cell homeostasis maintenance privilege eye. However mode or its activity eye not fully understood. In normal eyes, was detected epithelia iris body anterior region epithelium. expressed constitutively associated vesicular structures but detectable contrast, posterior RPE, which under conditions negative for strong staining molecules areas adjacent sub-retinal inflammatory infiltrates. Immunofluorescence Western blot analysis indicated ARPE-19 express soluble membrane form FasL. The intracellular concentration significantly increased grown presence interferon (INF)-γ. by previously shown be positive also Expression recombinant fluorescent construct together immuno-staining demonstrated localises endocytic compartment melanoma (positive control). devoid specialised functions (e g. HeLa cells) localised membrane, demonstrating RPE lysosomes. suggest vesicles. Both forms may contribute different ways effectiveness response privilege.