Effect of puerarin on retinal pigment epithelial cells apoptosis induced partly by peroxynitrite via Fas/FasL pathway.
作者： Yu-Hua Shen , Yan-Hua Wang , Zhi-Yun Wang , Yan-Qing Zhang , Li-Na Hao
DOI: 10.3980/J.ISSN.2222-3959.2010.04.01
关键词:
摘要: AIM To evaluate the peroxynitrite (ONOO(-)) of puerarin on retinal pigment epithelial (RPE) cells apoptosis induced partly by via Fas/FasL. METHODS RPE from C57BL/6 mice eyes were cultured. Diabetes was in Sprague-Dawley (SD) rats streptozotocin (STZ) intraperitoneal injection. Puerarin administrated to cultured and diabetic rats. Western blotting analysis, DNA ladder, RT-PCR, immunohistochemistry used for determining expression nitrotyrosine (NT, foot print ONOO(-)), complement 3 (C3); inducible nitric oxide synthase (iNOS) mRNA as well Fas/FasL signal transduction cells. RESULTS Both ONOO(-) group developed expressed NT, C3, iNOS But latter delayed all changes a time-dependent manner compared with control STZ (P<0.001). iNOS, C3 up-regulated associated an increase ONOO(-)in vivo vitro. CONCLUSION decreases retinopathy.
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