The effect of pre-analytical variables on light transmittance aggregometry in citrated platelet-rich plasma from healthy subjects.

摘要: Background: Although light transmittance aggregometry is considered the gold standard for platelet function testing, it poorly standardized. The effect of several pre-analytical variables on this assay was investigated. Methods: Light performed with an automated coagulation analyzer using arachidonic acid (1.6 mmol/L), adenosine-5-diphosphate (ADP) (11 mmol/L) and collagen mg/L, all final concentrations). results were reported as maximum aggregation (in %) in 10 min. Twenty apparently healthy subjects tested three times two consecutive days: day 1, fasting samples collected morning mid-day; 2, after a breakfast. immediately preparation platelet-rich-plasma (PRP), stabilization (30 min) non-adjusted count (225–275=10 9 /L) adjusted PRP, at 2 4 h blood collection. Results: Maximum higher nonadjusted compared to PRP agonists used (all p-0.05). Arachidonic ADP, but not collagen, induced maximal significantly decreased (arachidonic from 84%, 73%–90% 71%, 28%–85%, p-0.001; ADP 79%, 62%–87% 66%, 50%–79%, p-0.001, medians inter-quartile ranges). Short-term consumption breakfast sampling mid-day had no significant aggregation. Conclusions: Blood collection plasma can be simplified by omitting short-term adjustment count. mid-day, acceptable.