In vivo association of E2F and DP family proteins.
作者： C L Wu , L R Zukerberg , C Ngwu , E Harlow , J A Lees
关键词: Protein subunit 、 E2F1 Transcription Factor 、 E2F 、 Complementary DNA 、 E2F Transcription Factors 、 Transcription (biology) 、 Biology 、 Molecular biology 、 Plasma protein binding 、 Transcription factor
摘要: The mammalian transcription factor E2F plays an important role in regulating the expression of genes that are required for passage through cell cycle. This transcriptional activity is inhibited by association with retinoblastoma tumor suppressor protein (pRB) or its relatives p107 and p103. first cDNA from family to be cloned was designated E2F-1, multiple members have now been identified. They bind DNA as heterodimers, interacting proteins known DP. Here we demonstrate DP also a polypeptides at least two (hDP-1 hDP-2). Both hDP-1 hDP-2 all vivo, each complex capable activating transcription. However, various E2F/DP complexes display strong differences ability either pRB specificity binding mediated subunit.
sci-hub.se 参考文章(53)
C.W. Anderson, E. Harlow, J.A. Lees, K.J. Buchkovich, D.R. Marshak, The retinoblastoma protein is phosphorylated on multiple sites by human cdc2. The EMBO Journal. ,vol. 10, pp. 4279- 4290 ,(1991) , 10.1002/J.1460-2075.1991.TB05006.X
L.R. Bandara, V.M. Buck, M. Zamanian, L.H. Johnston, N.B. La Thangue, Functional synergy between DP-1 and E2F-1 in the cell cycle-regulating transcription factor DRTF1/E2F. The EMBO Journal. ,vol. 12, pp. 4317- 4324 ,(1993) , 10.1002/J.1460-2075.1993.TB06116.X
J-Y Kato, H Matsushime, SW Hiebert, ME Ewen, CJ Sherr, Direct binding of cyclin D to the retinoblastoma gene product (pRb) and pRb phosphorylation by the cyclin D-dependent kinase CDK4. Genes & Development. ,vol. 7, pp. 331- 342 ,(1993) , 10.1101/GAD.7.3.331
L.R. Bandara, E.W. Lam, T.S. Sørensen, M. Zamanian, R. Girling, N.B. La Thangue, DP-1: a cell cycle-regulated and phosphorylated component of transcription factor DRTF1/E2F which is functionally important for recognition by pRb and the adenovirus E4 orf 6/7 protein. The EMBO Journal. ,vol. 13, pp. 3104- 3114 ,(1994) , 10.1002/J.1460-2075.1994.TB06609.X
J.K. Schwarz, S.H. Devoto, E.J. Smith, S.P. Chellappan, L. Jakoi, J.R. Nevins, Interactions of the p107 and Rb proteins with E2F during the cell proliferation response. The EMBO Journal. ,vol. 12, pp. 1013- 1020 ,(1993) , 10.1002/J.1460-2075.1993.TB05742.X
S W Hiebert, M Blake, J Azizkhan, J R Nevins, Role of E2F transcription factor in E1A-mediated trans activation of cellular genes. Journal of Virology. ,vol. 65, pp. 3547- 3552 ,(1991) , 10.1128/JVI.65.7.3547-3552.1991
David Lane, Ed Harlow, Antibodies: A Laboratory Manual ,(1988)
Q J Hu, C Bautista, G M Edwards, D Defeo-Jones, R E Jones, E Harlow, Antibodies specific for the human retinoblastoma protein identify a family of related polypeptides. Molecular and Cellular Biology. ,vol. 11, pp. 5792- 5799 ,(1991) , 10.1128/MCB.11.11.5792
P A Hamel, R M Gill, R A Phillips, B L Gallie, Transcriptional repression of the E2-containing promoters EIIaE, c-myc, and RB1 by the product of the RB1 gene. Molecular and Cellular Biology. ,vol. 12, pp. 3431- 3438 ,(1992) , 10.1128/MCB.12.8.3431
H Matsushime, D E Quelle, S A Shurtleff, M Shibuya, C J Sherr, J Y Kato, D-type cyclin-dependent kinase activity in mammalian cells. Molecular and Cellular Biology. ,vol. 14, pp. 2066- 2076 ,(1994) , 10.1128/MCB.14.3.2066