In vitro hemocompatibility studies of drug-loaded poly-(L-lactic acid) fibers

摘要: Our objective was to evaluate the hemocompatibility of biodegradable stent fibers, employing a closed-loop circulation system filled with human blood. We also investigated effects anti-inflammatory and anti-proliferative drugs curcumin paclitaxel, incorporated into fibers. Fresh whole blood circulated in four parallel systems: empty tube circuit (control) tubes containing either PLLA fiber coil (PLLA), curcumin-loaded (C-PLLA) or paclitaxel-loaded (P-PLLA). The influence fiber, alone loaded drug during melt-extrusion, on leukocyte platelet adhesion activation determined by flow cytometry. properties cell deposition were assessed scanning electron microscopy (SEM). cytometry results clearly demonstrated that triggers at site deployment, as shown increases CD11b, CD62P leukocyte-platelet aggregates, compared controls. Curcumin paclitaxel treatments both significantly reduced SEM. Activated leukocytes platelets revealed lower CD11b receptor binding for C-PLLA alone, slightly P-PLLA. Reductions platelet-leukocyte aggregates observed well. In addition, there less C-PLLA, controls, A continuous linear thrombus, composed platelets, leukocytes, red cells fibrin occasionally detected along line tangency between wall. Flow separation eddying, proximal distal tube, is thought contribute this deposit. more effective than reducing fibers setting. However evidence degeneration melt extrusion may have inhibited its effectiveness. Incorporation bioresorbable proposed prevent thrombosis in-stent restenosis.