Identification of the gene and mRNA for the adenovirus terminal protein precursor

摘要: The precursor of the 55K adenovirus terminal protein is an 87K that covalently linked to viral DNA. This likely be identical 80,000 dalton described by Challberg et al. (1980). mRNA for precursor, like E2-72K DNA binding protein, detectable at both early and late times infection, its production sensitive synthesis inhibition (Lewis Mathews, 1980). together with proteins 105,000 75,000 daltons, are translated from leftward transcribed (1-strand) messenger RNAs complementary genome between positions 11.2 31.5. Additional hybridization region coordinates 37.3 41 suggests RNA body spliced sequences mapping farther right in genome. Electron microscopic heteroduplex analysis has revealed a family 1-strand probably encode these proteins. bodies extend coordinated 30, 26 23 11.1, leaders 39, 68.5 75 map units, defining new region. These E2 share first leader presumably same promoter, may coordinately expressed. Virions protease-deficient 2 mutant ts1 grown restrictive temperature contain only form; when permissive they forms, additional 62K wild-type virions form. Peptide shows all related. DNA-protein complex containing form active as template replication vitro. data supports model which primary translation product primes synthesis. processed curing virus maturation possibly via intermediate form, virus-specified Ad2ts1 protease.