Early Events of Virus-Cell Interaction in an Adenovirus System

摘要: The interaction of (32)P-labeled adenovirus type 2 and HeLa or KB cells has been examined during early infection. kinetics virus uncoating to deoxyribonuclease-sensitive products, the partial characterization three such products by gradient centrifugation, distribution these in extranuclear nuclear portions infected are reported. results compatible with following model. Extracellular attaches a receptor on plasma membrane. membrane-bound half-life less than 15 min is transformed partly uncoated product which free inside cell about half rapidly enters nucleus. This transformed, both cytoplasm nucleus, virion "core." proteins bound "core" then removed from intact deoxyribonucleic acid (DNA). In viral DNA main there overall sequence completed hr.