Intracellular uncoating of type 5 adenovirus deoxyribonucleic acid.

摘要: Highly purified, 32 P-labeled type 5 adenovirus was employed to study “uncoating” of viral deoxyribonucleic acid (DNA)—defined as the development sensitivity deoxyribonuclease. Viral infectivity and radioactivity adsorbed KB cells at same rate, significant amounts P did not elute from throughout eclipse period. Kinetic studies penetration, infectivity, uncoating DNA indicated that three events were closely related temporally, rates each similar, they completed within 60 90 min after infection. eclipse, proceeded normally under conditions which blocked protein synthesis, but occur 0 4 C. Neither nor degraded acid-soluble material during The nature studied it converted intracellularly deoxyribonuclease-resistant deoxyribonuclease-susceptible. Intact virions centrifuged in sucrose gradients had a sedimentation coefficient approximately 800, sedimented particle about 30 S . Infection with purified virus yielded deoxyribonuclease-susceptible nucleic particles coefficients 350 450 , i.e., greater than 10 times faster obtained been disrupted by exposure p H 10.5. When mixed cell lysates, its characteristics essentially unchanged presence cellular material.