The intracellular fate of rabbitpox virus rendered noninfectious by various reagents

摘要: Abstract Highly purified and radioactively-labeled rabbitpox virus was treated with various reagents calculated to modify selectively viral DNA or protein. In each instance the following were examined: conversion of particles cores; uncoating cores naked in cells containing intact virions; stability inducer protein, as measured by ability noninfectious induce formation As is exposed ultraviolet (UV) light, infectivity abolished first, followed shortly inactivation The be degraded cores, such uncoated which protein present, are resistant UV light. Rabbitpox inactivated methylation methylmethanesulfonic acid visible light after interaction neutral red behaves similarly UV-light. Virus 5-bromouracil moiety apparently prematurely within vesicles taken up into cells. progressively heat, there appear but still intact; however, this quickly heating continued. Urea inactivates when present concentrations exceeding 4 M . Virus-antibody complexes adsorbed, majority elute. Those that not DNA. Virusantibody cause profound changes surface they adsorb. Wherever possible, information gained concerning biological activity related phenomenon poxvirus reactivation.